Metode standard plate count pdf

Diagram alir proses penentuan frekuensi hpef dapat dilihat pada gambar 11. Two methods of growing is usually practiced the pour plate or the spread plate. Dye reduction time, inversely proportional to the number of cells in the sample, causes a color change from blue to white methylene blue or slate bluepink to white resazurin. That is, the final plates in the series should have between 30 and 300 colonies. Usfda 2001 dengan pipet steril, sampel diambil dari campuran yang sudah disiapkan dengan pengenceran 102, 103, 104 dan pengenceran lain dengan mengambil 10 ml pengenceran sebelumnya ke dalam 90 ml larutan pengencer. Kelompok 3b ade yuliani i14080012 nining tyas tri atmaja i14080024 dian rizki eka rizal i14080060 trikorian ade sanjaya i14080093 ika meilaty i14080120. Define limitations for standard or total plate counts method. Pdf lab report of microbiology akbar haqi academia. The most commonly used microbiological count method is the standard plate count spc agar method. The significance of spcs, however, varies markedly according to the type of food product and the processing it has received.

Media plate count agar pca medical laboratory technologist. Standard methods agar for the plate count procedure. Sc 2, aris sri widati 2 1 mahasiswa bagian teknologi hasil ternak fakultas peternakan, universitas brawijaya, malang, 65145, indonesia 2 dosen bagian teknologi hasil ternak fakultas peternakan, universitas brawijaya, malang. The 3mtm petrifilm tm aerobic count ac plate is a samplereadyculture. Mechanical shakers may be used only if a laboratory provides validation data on a specific unit. The complete study was designed for a period of 12 months from. Standard plate count background the standard plate count spc is used as an indicator of the level of bacteria in dairy products. Introduction microorganism is an organism that is microscopic or submicroscopic, which is too small to be seen under naked eyes. Meskipun kedua metode tersebut kadang akan menghasilkan hasil perhitungan yang mirip, tetapi keduanya memiliki perbedaan prinsip. Hal tersebut bergantung pada jumlah dan jenis mikroorganisme yang ada dalam makanan atau minuman tersebut.

Analisis cemaran bakteri coliform escherichia coli coli. Prinsip metode cawan hitung plate count adalah jika sel mikroba yang masih hidup ditumbuhkan pada medium agar, maka sel mikroba tersebut akan berkembang biak dan membentuk koloni yang dapat dilihat lansung dengan mata tanpa menggunakan mikroskop. Microbiological standard for processed food in indonesia is regulated by the head of nadfc in decree no. Depending on the source of the sample used there might be thousands, millions or even billions of microorganisms per millilitre of sample. Plate count agarstandard methods agar tryptone glucose.

Form fdancims 2400a standard plate count and coliform plate count rev. Namun, ada dua metode yang paling sering digunakan yaitu metode hitung koloni di cawan petri standard viable plate count method dan analisa spektrofotometer turbidimeter. Diss olve in gred ients at 95 o c, repipet into 16 x 150 mm tubes, cap, autoclave, 15 lb, 15 min. Total plate count of milk from dairy cooperatives in. Uji total plate count tpc dan enterobacter daging kambing di pasar kota malang sufyan arif 1, masdiana ch. A statistical comparison of the spiral plate count splpc and the standard plate count spc methods for enumeration. Fewer than 30 colonies are not acceptable for statistical. Spc adalah suatu metode yang digunakan untuk menentukan kerapatan bakteri aerob dan aerob fakultatif heterotrof dalam air, perhitungan ini menggunakan asumsi.

Pemeriksaan kualitas air dan makanan dilakukan untuk mengetahui layak atau tidaknya makanan atau minuman tersebut kita konsumsi. Fastidious microorganisms may not be able to grow on the agar medium used. Ii pengujian total mikroba metode standard plate count oleh. Pemeriksaan ini dapatdilakukan dengan metode tpc total plate count, yaitu dengan menghitung jumlah. Apa yang anda ketahui mengenai standard plate count. Metode ini dilakukan dengan mengencerkan sumber isolate yang telah diketahui beratnya ke dalam 9 ml larutan garam fisiologis, larutan yang digunakan sekitar 1 ml suspense ke dalam cawan petri steril, dilanjutkan dengan menuangkan media penyubur nutrient agar, na media penyubur merupakan nutrisi.

Development and validation of a microbial counting. Ada dua metode yang umum digunakan dalam plate count, yaitu. Nearly 700 standard plate count spc bacteria were isolated from drinking water and untreated surface water and identified according to a scheme developed to permit the rapid, simple classification of microorganisms to genus, species, or group. Most probable number an overview sciencedirect topics. In foods that do not contain many inherent reductive compounds, the dye reduction assay is comparable with the standard plate count method.

Microbial count depends on the type of media, incubation temperature, incubation time, distribution of the organism in the food sample and sampling method used. Proses penghitungan sel bakteri dapat dilakukan dengan beberapa metode baik secara langsung maupun tidak langsung, diantaranya adalah metode hitung pada cawan petri standard plate count, metode pengamatan langsung dengan kaca objek atau metode hitung dengan menggunakan haemocytometer, metode ukur kekeruhan turbidimetri menggunakan. The purpose, as its name implies, is to estimate the number of viable microorganism cells in a given sample of food. A comparison of pour plate and spread plate methods. Define limitations for standard or total plate counts. Purpose this document describes the titration methods used by the bacteriology section of the center for veterinary biologics cvb to determine the colonyforming units cfu in final container samples. Standard plate count the standard plate count spc, also referred to as the aerobic plate count or the total viable count, is one of the most common tests applied to indicate the microbiological quality of food. A comparison of pour plate and spread plate methods article pdf available in journal american water works association 751. The dilution series is continued by using a new pipette to transfer a 1. Standard methods agar plate count agar for standard. Water samples collected during routine flushing of deadend mains were analyzed for bacterial count by using both pour plate and spread plate procedures.

Typically this agar consists of peptone, yeast extract glucose and agar. Plate count agar pca atau yang juga sering disebut dengan standard methods agar sma merupakan sebuah media pertumbuhan mikroorganisme yang umum digunakan untuk menghitung jumlah bakteri total semua jenis bakteri yang terdapat pada setiap sampel seperti makanan, produk susu, air limbah dan sampelsampel lainnya yang juga biasanya menggunakan metode. Enumeration and characterization of standard plate count. However, the numbers of microorganisms in a given sample are required to know in certain aspect such as dairy industries, diseases investigation, and so on. For example, if we counted 32 bacteria on a plate that had received 200. Standard methods agar is a modified formulation of tryptone glucose skim milk agar that was developed by bowers and hucker. Plate count agar is a medium used for the enumeration of bacteria in food, water and other materials of sanitary. Plate count agar standard methods agar m091 intended use recommended for the determination of plate counts of microorganisms in food, water, waste water and also from clinical samples.

Heterotrophic plate counts and drinkingwater safety. This is too many for us to count so we dilute the sample. Penelitian utama penelitian utama meliputi aplikasi kombinasi metode uv dan hpef dalam menginaktivasi bakteri uji yaitu staphylococcus aureus atcc 25923 dan escherichia coli atcc 25922 yang direkontaminasi pada susu segar yang telah disterilisasi. Results from these analyses were compared statistically. The 12th edition of standard methods for the examination of water and wastewater states that the isolation of pathogenic bacteria.

Pdf enumeration and characterisation of standard plate count. Average the count of the duplicate plates, multiply by the dilution factor and record as the number of bacteria. Analisis kuantitatif mikrobiologi pada makanan penerbangan. Chapter 6 microbiological count methods standard methods. This microbiological growth medium is not a selective medium, arasains indonesia provides biolife standard plate count agar. Many translated example sentences containing standard plate count french english dictionary and. Perhitungan total mikroba metode apc aerobic plate count, pour plate bacteriological analytical manual online. Tes jurnal 5 tes bakteri total metode standard plate count. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Standard methods agar is equivalent to the formulation of plate count agar tryptone glucose yeast agar as.

Cow milk is more widely consumed than that of horse or goat because of the people habit and the raw milk is more easily found in the market. Total plate count can be made using plate count agar. Standard operating policyprocedure page 3 of 16 standard bacterial plate count uncontrolled copy 1. Spc standard plate count kl kaki lima d delivery apm angka partisipasi murni emb eosin methylene blue. In order to obtain a validated method for microbial counting and to ensure its safety and reliability within.

Pdf nearly 700 standard plate count spc bacteria were isolated from drinking water and untreated surface water and identified according to. Standard operating policy procedure page 6 of 16 standard bacterial plate count uncontrolled copy 4. Count the number of colonies on those plates showing 25250 colonies note 4. Beberapa cara dapat digunakan untuk menghitung atau mengukur jumlah jasad renik didalam suatu suspensi atau bahan yaitu dengan perhitungan jumlah sel salah satunya dengan metode hitungan cawan dan dianalisis berdasarkan spc standard plate count. Pada metode tuang, sejumlah sampel 1ml atau 0,1ml dari pengenceran yang dikehendaki dimasukkan kecawan petri, kemudian ditambah agaragar cair steril yang didinginkan 4750 o c sebanyak 1520 ml dan digoyangkan. To compute the heterotrophic plate count, cfuml, multiply total number of colonies or average number if duplicate plates of the same dilution per plate by the reciprocal of the dilution used. Metode hitungan cawan dibedakan atas dua cara, yakni metode tuang pour plate, dan metode permukaan surface spread plate. The standard plate count method consists of diluting a sample with sterile saline or phosphate buffer diluent until the bacteria are dilute enough to count accurately. Highly differential in which we can be almost certain that colonies that meet the description belong to. Enumeration and identification of standard plate count. Total plate count of milk from dairy cooperatives in yogyakarta and east java most of the local populations in indonesia, especially in sleman, yogyakarta and tulungagung, east java consume cow milk. Because of this, a variety of methods have been developed.